t 624 vaginalis atcc 30236  (ATCC)

Journal: Frontiers in Parasitology

Article Title: In vitro co-culture model of Trichomonas vaginalis , Candida albicans , and Lactobacillus crispatus : a system for assessing antimicrobial activity and microorganism interactions in vaginitis

doi: 10.3389/fpara.2025.1523113

Figure Lengend Snippet: Initial densities of ATTC 30236 Trichomonas vaginalis isolate (TV), Candida albicans (CA) and Lactobacillus crispatus (LC) for Checkerboard assays.

Article Snippet: For C. albicans biofilm biomass, co-culture with the ATCC T. vaginalis isolate (ATCC30236, initial density 1.00x10 6 trophozoites/mL) led to a 60% reduction, with biofilm decreasing to 40%).A more pronounced reduction (67%) was observed in the triple co-culture (ATCC30236, CA3, and LC2), resulting in a biofilm biomass of 33%).In the case of L. crispatus , co-culture with ATCC30236 led to a 96% reduction in total biofilm biomass (remaining biomass 4.0%), while the triple co-culture reduced biomass by 68% (remaining biomass 32%).

Techniques:

Journal: Frontiers in Parasitology

Article Title: In vitro co-culture model of Trichomonas vaginalis , Candida albicans , and Lactobacillus crispatus : a system for assessing antimicrobial activity and microorganism interactions in vaginitis

doi: 10.3389/fpara.2025.1523113

Figure Lengend Snippet: The mean viability of TV-LACM6 Trichomonas vaginalis isolate, ATCC 2433 Candida albicans isolate, and Lactobacillus crispatus isolate after 24h of co-culture.

Article Snippet: For C. albicans biofilm biomass, co-culture with the ATCC T. vaginalis isolate (ATCC30236, initial density 1.00x10 6 trophozoites/mL) led to a 60% reduction, with biofilm decreasing to 40%).A more pronounced reduction (67%) was observed in the triple co-culture (ATCC30236, CA3, and LC2), resulting in a biofilm biomass of 33%).In the case of L. crispatus , co-culture with ATCC30236 led to a 96% reduction in total biofilm biomass (remaining biomass 4.0%), while the triple co-culture reduced biomass by 68% (remaining biomass 32%).

Techniques:

Journal: Frontiers in Parasitology

Article Title: In vitro co-culture model of Trichomonas vaginalis , Candida albicans , and Lactobacillus crispatus : a system for assessing antimicrobial activity and microorganism interactions in vaginitis

doi: 10.3389/fpara.2025.1523113

Figure Lengend Snippet: Growth kinetic curves: (A) ATCC 30236 Trichomonas vaginalis isolate in monoculture or co-culture with Candida albicans at 3.3 x 10 4 CFU/mL (CA3) and Lactobacillus crispatus at 5.53 x 10 6 CFU/mL (LC2). Co-cultured trophozoites were compared with control (monocultured); (B) TV-LAM6 T. vaginalis isolate in monoculture or co-culture with CA3 and L. crispatus at 5.53 x 10 7 CFU/mL (LC1). Co-cultured trophozoites were compared with control (monocultured). (C) C. albicans in monoculture (CA3), L. crispatus in monoculture (LC2). Data are the mean ± S. D. of at least three different experiments performed in triplicate. *Means statistical difference from control ( P < 0.05).

Article Snippet: For C. albicans biofilm biomass, co-culture with the ATCC T. vaginalis isolate (ATCC30236, initial density 1.00x10 6 trophozoites/mL) led to a 60% reduction, with biofilm decreasing to 40%).A more pronounced reduction (67%) was observed in the triple co-culture (ATCC30236, CA3, and LC2), resulting in a biofilm biomass of 33%).In the case of L. crispatus , co-culture with ATCC30236 led to a 96% reduction in total biofilm biomass (remaining biomass 4.0%), while the triple co-culture reduced biomass by 68% (remaining biomass 32%).

Techniques: Co-Culture Assay, Cell Culture, Control

Journal: Frontiers in Parasitology

Article Title: In vitro co-culture model of Trichomonas vaginalis , Candida albicans , and Lactobacillus crispatus : a system for assessing antimicrobial activity and microorganism interactions in vaginitis

doi: 10.3389/fpara.2025.1523113

Figure Lengend Snippet: pH values in monocultures and co-cultures of Lactobacillus crispatus , Candida albicans and Trichomonas vaginalis .

Article Snippet: For C. albicans biofilm biomass, co-culture with the ATCC T. vaginalis isolate (ATCC30236, initial density 1.00x10 6 trophozoites/mL) led to a 60% reduction, with biofilm decreasing to 40%).A more pronounced reduction (67%) was observed in the triple co-culture (ATCC30236, CA3, and LC2), resulting in a biofilm biomass of 33%).In the case of L. crispatus , co-culture with ATCC30236 led to a 96% reduction in total biofilm biomass (remaining biomass 4.0%), while the triple co-culture reduced biomass by 68% (remaining biomass 32%).

Techniques:

Journal: Frontiers in Parasitology

Article Title: In vitro co-culture model of Trichomonas vaginalis , Candida albicans , and Lactobacillus crispatus : a system for assessing antimicrobial activity and microorganism interactions in vaginitis

doi: 10.3389/fpara.2025.1523113

Figure Lengend Snippet: Minimum inhibitory concentrations (MIC) of metronidazole for Trichomonas vaginalis , minimum fungicidal concentration MFC) of Fluconazole for Candida albicans , and minimum bactericidal concentration (MBC) of streptomycin/penicillin for Lactobacillus crispatus : Comparative analysis of MIC, MBC, and MFC values and mean trophozoites/mL or CFU/mL after 24 h in monocultures and co-cultures.

Article Snippet: For C. albicans biofilm biomass, co-culture with the ATCC T. vaginalis isolate (ATCC30236, initial density 1.00x10 6 trophozoites/mL) led to a 60% reduction, with biofilm decreasing to 40%).A more pronounced reduction (67%) was observed in the triple co-culture (ATCC30236, CA3, and LC2), resulting in a biofilm biomass of 33%).In the case of L. crispatus , co-culture with ATCC30236 led to a 96% reduction in total biofilm biomass (remaining biomass 4.0%), while the triple co-culture reduced biomass by 68% (remaining biomass 32%).

Techniques: Concentration Assay, Co-Culture Assay

Journal: Frontiers in Parasitology

Article Title: In vitro co-culture model of Trichomonas vaginalis , Candida albicans , and Lactobacillus crispatus : a system for assessing antimicrobial activity and microorganism interactions in vaginitis

doi: 10.3389/fpara.2025.1523113

Figure Lengend Snippet: Cytolysis measured by LDH release from human vaginal epithelial cells co-incubated with monocultures or co-cultures of Trichomonas vaginalis, Candida albicans and Lactobacillus crispatus. (A) Monocultures of L. crispatus (LC2, 5.53 x 10 6 CFU/mL) C. albicans (CA3, 3.33 x 10 4 CFU/mL), and T. vaginalis standard isolate (ATCC30236, 1.0 x 10 6 trophozoites/mL). Dual co-cultures: LC2 + CA3, ATC30236 + CA3, and ATCC30236 + LC2. Triple co-culture: ATCC30236 + CA3 + LC2. (B) Monocultures of L. crispatus (LC1, 5.53 x 10 7 CFU/mL), C albicans (CA3), and T. vaginalis fresh clinical isolate (TV-LACM6, 1.0 x 10 6 trophozoites/mL). Dual co-cultures: LC1 + CA3, TV-LACM6 + CA3, and TV-LACM6 + LC1. Triple co-culture: TV-LACM6 + CA3 + LC1. Positive control of cytolysis is HMVII cells treated with 0.2% Triton X-100. Results are expressed as a percentage of total cytolysis. Data are presented as the mean ± S.D. of at least two experiments. The percentage of LDH released from HMVII cells with the monocultures of T. vaginalis isolates was compared with that from HMVII cells with co-cultures with the protozoan. (*) indicates a significant difference.

Article Snippet: For C. albicans biofilm biomass, co-culture with the ATCC T. vaginalis isolate (ATCC30236, initial density 1.00x10 6 trophozoites/mL) led to a 60% reduction, with biofilm decreasing to 40%).A more pronounced reduction (67%) was observed in the triple co-culture (ATCC30236, CA3, and LC2), resulting in a biofilm biomass of 33%).In the case of L. crispatus , co-culture with ATCC30236 led to a 96% reduction in total biofilm biomass (remaining biomass 4.0%), while the triple co-culture reduced biomass by 68% (remaining biomass 32%).

Techniques: Incubation, Co-Culture Assay, Positive Control

Journal: Frontiers in Parasitology

Article Title: In vitro co-culture model of Trichomonas vaginalis , Candida albicans , and Lactobacillus crispatus : a system for assessing antimicrobial activity and microorganism interactions in vaginitis

doi: 10.3389/fpara.2025.1523113

Figure Lengend Snippet: Hemolysis of erythrocytes co-incubated with monocultures or co-cultures of Trichomonas vaginalis, Candida albicans and Lactobacillus crispatus. (A) ATCC30236 T. vaginalis standard isolate, C. albicans (CA3), and L. crispatus (LC2). (B) TV-LACM6 T. vaginalis fresh clinical isolate, C. albicans (CA3), and L. crispatus (LC1). Positive control of hemolysis is erythrocytes treated with 0.2% Triton X-100. Results are expressed as a percentage of total hemolysis, presented as the mean ± S.D. of at least two blood samples. The percentage of hemolysis from erythrocytes co-incubated with T. vaginalis monocultures was compared to co-cultures with the protozoan. (*) indicates a significant difference.

Article Snippet: For C. albicans biofilm biomass, co-culture with the ATCC T. vaginalis isolate (ATCC30236, initial density 1.00x10 6 trophozoites/mL) led to a 60% reduction, with biofilm decreasing to 40%).A more pronounced reduction (67%) was observed in the triple co-culture (ATCC30236, CA3, and LC2), resulting in a biofilm biomass of 33%).In the case of L. crispatus , co-culture with ATCC30236 led to a 96% reduction in total biofilm biomass (remaining biomass 4.0%), while the triple co-culture reduced biomass by 68% (remaining biomass 32%).

Techniques: Incubation, Positive Control

Journal: Frontiers in Parasitology

Article Title: In vitro co-culture model of Trichomonas vaginalis , Candida albicans , and Lactobacillus crispatus : a system for assessing antimicrobial activity and microorganism interactions in vaginitis

doi: 10.3389/fpara.2025.1523113

Figure Lengend Snippet: Biofilm formation and viability of Candida albicans and Lactobacillus crispatus in monoculture and co-culture (A) C. albicans (CA3, 3.33x10 4 CFU/mL), and second initial density of L. crispatus (LC2, 5.53x10 6 CFU/mL) in monoculture or co-culture. Co-culture with ATCC T. vaginalis isolate (ATCC30236, 1 x 10 6 trophozoites/mL) including the combinations ATCC30236 + CA3, ATCC30236 + LC2, and ATCC30236 + CA3 + LC2. 1 Percentage represent biofilm biomass and biofilm viability of ATCC30236 + LC2, respectively. (B) Biofilm formation and viability by CA3, and first initial density of L. crispatus (LC1, 5.53x10 7 CFU/mL) in monoculture or co-culture (CA3 + LC1), and in co-culture with fresh clinical T. vaginalis isolate (TV-LACM6) including the combinations: TV-LACM6 + CA3, TV-LACM6 + LC1, and TV-LACM6 + CA3 + LC1. (a) Statistically significant difference in biofilm viability (p < 0.05). (*) Statistically significant difference in biofilm biomass formation (p < 0.05).

Article Snippet: For C. albicans biofilm biomass, co-culture with the ATCC T. vaginalis isolate (ATCC30236, initial density 1.00x10 6 trophozoites/mL) led to a 60% reduction, with biofilm decreasing to 40%).A more pronounced reduction (67%) was observed in the triple co-culture (ATCC30236, CA3, and LC2), resulting in a biofilm biomass of 33%).In the case of L. crispatus , co-culture with ATCC30236 led to a 96% reduction in total biofilm biomass (remaining biomass 4.0%), while the triple co-culture reduced biomass by 68% (remaining biomass 32%).

Techniques: Co-Culture Assay

Journal: Frontiers in Parasitology

Article Title: In vitro co-culture model of Trichomonas vaginalis , Candida albicans , and Lactobacillus crispatus : a system for assessing antimicrobial activity and microorganism interactions in vaginitis

doi: 10.3389/fpara.2025.1523113

Figure Lengend Snippet: Yeast-to-hyphal form transition of Candida albicans in monoculture and co-culture. (A) Co-culture of C. albicans (CA3, initial density at 3.33x10 4 CFU/mL) with ATCC Trichomonas vaginalis isolate (ATCC3026, initial density at 1 x 10 6 trophozoites/mL) and second density of Lactobacillus crispatus (LC2, i nitial density at 5.53x10 6 CFU/mL). (B) Co-culture of CA3 with fresh clinical T. vaginalis isolate (TV-LACM6, initial density at 1 x 10 6 trophozoites/mL) and first density of L. crispatus (LC1, initial density at 5.53x10 7 CFU/mL. The date were expressed by percentage de hyphae formation. Results are representative of two independent experiments conducted with triplicate assays. (*) Statistically significant difference (p < 0.05).

Article Snippet: For C. albicans biofilm biomass, co-culture with the ATCC T. vaginalis isolate (ATCC30236, initial density 1.00x10 6 trophozoites/mL) led to a 60% reduction, with biofilm decreasing to 40%).A more pronounced reduction (67%) was observed in the triple co-culture (ATCC30236, CA3, and LC2), resulting in a biofilm biomass of 33%).In the case of L. crispatus , co-culture with ATCC30236 led to a 96% reduction in total biofilm biomass (remaining biomass 4.0%), while the triple co-culture reduced biomass by 68% (remaining biomass 32%).

Techniques: Co-Culture Assay

Journal: Frontiers in Parasitology

Article Title: In vitro co-culture model of Trichomonas vaginalis , Candida albicans , and Lactobacillus crispatus : a system for assessing antimicrobial activity and microorganism interactions in vaginitis

doi: 10.3389/fpara.2025.1523113

Figure Lengend Snippet: Scanning electron microscopy of ATCC 30236 and TV-LACM6 isolates in monoculture or co-culture with ATCC 24433 C. albicans and CCT 7595 L. crispatus. Left side: Co-culture of the T. vaginalis ATCC30236 isolate (initial density 1.00x10 6 trophozoites/mL, red arrow), C albicans CA3 (initial density 3.3x10 4 CFU/mL, orange arrow) and L. crispatus LC2 (initial density 5.53x10 6 CFU/mL, white arrow). Right side: Co-culture of fresh clinical isolate TV-LACM6 (initial density 1.00x10 6 trophozoites/mL, blue arrow), C albicans CA3 (orange arrow), and L. crispatus LC2 (initial density 5.53x10 6 CFU/mL, white arrow).

Article Snippet: For C. albicans biofilm biomass, co-culture with the ATCC T. vaginalis isolate (ATCC30236, initial density 1.00x10 6 trophozoites/mL) led to a 60% reduction, with biofilm decreasing to 40%).A more pronounced reduction (67%) was observed in the triple co-culture (ATCC30236, CA3, and LC2), resulting in a biofilm biomass of 33%).In the case of L. crispatus , co-culture with ATCC30236 led to a 96% reduction in total biofilm biomass (remaining biomass 4.0%), while the triple co-culture reduced biomass by 68% (remaining biomass 32%).

Techniques: Electron Microscopy, Co-Culture Assay